普诺赛NCI-H146 [H146] (人小细胞肺癌细胞)

产品目录

产品信息

名称	NCI-H146 [H146] (人小细胞肺癌细胞) (STR鉴定正确)
别称	H146; H-146; NCIH146
背景描述	Derived from the pleural fluid of a patient with small cell lung cancer; the cells express elevated levels of the 4 biochemical markers characteristic of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa decarboxylase and bombesin-like immunoreactivity; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the cells express relatively high amounts of c-myc mRNA, but no amplification of the gene is detected.
种属	人类
年龄（性别）	男性，59岁
组织来源	肺
生长特性	悬浮细胞
细胞形态	淋巴母细胞样
细胞类型	肿瘤细胞
肿瘤类型	肺癌细胞
生长培养基	RPMI-1640＋10% FBS＋1% P/S
推荐传代比例	1:2-1:3
推荐换液频率	2~3次/周
冻存条件	冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮
培养条件	气相：空气，95%；CO2，5%温度：37℃
基因表达情况	vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes -
注意事项	该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。
保藏机构	ATCC; HTB-173

暂无内容...