Dynabeads™ Untouched™ Human T Cells Kit
- 品名： Dynabeads™ Untouched™ Human T Cells Kit
- 品牌： 赛默飞世尔
- 型号： 暂无
- 产品详情
- 规格参数
Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched T cells
• Easy to use and to scale up
• No columns required
Methodology:
An antibody mix towards the non-T cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in a flow cytometer and used in any application.
Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended
Starting Sample:
PBMC
For Research Use Only. Not for use in diagnostic procedures.
Achieve functional knockout in human primary T-cells
| 容量: | Processes |
|---|---|
| Cell Type: | T Cells (Whole Population) |
| Final Product Type: | Cells |
| Isolation Technology: | Negative Isolation |
| 页反应的: | 100 Isolations |
| Output Viability: | >95% |
| Product Line: | DYNAL®, Dynabeads®, Untouched™ |
| Shipping Condition: | Room Temperature |
| 种类种类: | Human |
| Starting Material Cell No.: | 1x10^7 PBMCs per isolation |
| Target Species: | Human |
| 样品类型: | PBMC |
